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Z. Naturforsch. 2012,
67b, 944 – 950
doi:10.5560/ZNB.2012-0039
Structure Elucidation of Submilligram Quantities of Natural Products – Application to Haliclamines G and H from the Arctic Marine Sponge Haliclona viscosa
Alfred-Wegener-Institut für Polar- und Meeresforschung in der Helmholtz-Gemeinschaft, Am Handelshafen 12, 27570 Bremerhaven, Germany
Reprint requests to PD Dr. Matthias Köck. Fax: +49 471 4831 1425. E-mail:
mkoeck@awi.de
Received February 7, 2012 / published online September 20, 2012
Two new haliclamines were identified directly from the crude extract of the Arctic sponge Haliclona viscosa using improved chromatographic conditions and a detailed knowledge about the fragmentation pattern of haliclamines. These are haliclamine G (1) with two alkyl chains of equal length (10 methylene groups) and haliclamine H (2) with alkyl chains of 10 and 12 methylene groups. Due to the limited amount of sponge material available, the haliclamines were not isolated, and the structure elucidation relied on the chromatographic and mass spectrometric comparison with synthetic compounds.
Key words: Marine Sponges, Haliclona viscosa, 3-Alkyl Pyridine Alkaloids, Mass Spectrometry, Fragmentation Pattern
Supporting Material: